Notes Photosynthesis ICSE Class 10 Biology

Study Material

Students should refer to Photosynthesis ICSE Class 10 Biology notes provided below designed based on the latest syllabus and examination pattern issued by ICSE. These revision notes are really useful and will help you to learn all the important and difficult topics. These notes will also be very useful if you use them to revise just before your Biology Exams. Refer to more ICSE Class 10 Biology Notes for better preparation.

ICSE Class 10 Biology Photosynthesis Revision Notes

Students can refer to the quick revision notes prepared for Chapter Photosynthesis in Class 10 ICSE. These notes will be really helpful for the students giving the Biology exam in ICSE Class 10. Our teachers have prepared these concept notes based on the latest ICSE syllabus and ICSE books issued for the current academic year. Please refer to Chapter wise notes for ICSE Class 10 Biology provided on our website.

Photosynthesis ICSE Class 10 Biology

Quick Review
➢ Photosynthesis is the process in which green plants or their parts produce complex carbon containing compounds with the help of inorganic raw materials such as CO2 and water in the presence of sunlight. O2 is Liberated as the bi-product.

Notes Photosynthesis ICSE Class 10 Biology

The whole of the oxygen liberated comes from water, by a process called photolysis of water.
➢ This is an anabolic, endergonic (requires energy) & oxidation-reduction process. Photosynthesis is the only process by which solar energy is converted into chemical energy.
➢ A chloroplast is an oval, minute, double membrane organelle present in green plants. It contains a green pigment called chlorophyll which absorbs light energy for photosynthesis. Internally it contains a matrix or stroma and the thylakoids. At some places the thylakoids are arranged themselves to form a stalk of coins like structure called grana.
Each stalk of thylakoid sacs are connected by a structure known as stromal lamellae. The chloroplast mainly occurs in chlorenchymatous cells of leaves.
➢ Stomata are the structures mainly responsible for the gaseous exchange in the process of photosynthesis, respiration and transpiration.
➢ Stomata open in light when guard cells are turgid and close when they are flaccid. These turgor changes leading to the opening and closing of stomata.
➢ According to Malate K+ ions pump hypothesis, the turgor changes that open and close the stomata are due to the reversible absorption and loss of potassium ions ( K+).
➢ The stomata open when guard cells absorb K+ ions from surrounding epidermal cells. The intake of K+ ions by guard cells takes place through H+ and K+ ion exchange. The H+ ions appear in the guard cell due to dissociation of maleic acid. The maleic acid dissociate into malate anions and H+ ions in the guard cells.

Notes Photosynthesis ICSE Class 10 Biology

➢ During night, the K+ ion concentration decreases in the guard cells. This lowers the osmotic pressure of the guard cells which results in the closing of stomata due to exosmosis.
➢ Mechanism of photosynthesis : Photosynthesis consists of two stages – The light phase and the dark phase. In the light phase, light is absorbed and used by chlorophyll. This, therefore, is called the photochemical reaction or Hill’s reaction. It can be described in two steps – Photolysis and formation of assimilatory powers.
➢ Photolysis is the process of splitting of water in the presence of light. Water splits into H+ and OH ions. H+ ions go to NADP+ while OH ions split into water, oxygen and electron in the presence of Z complex enzymes, Mn and Clions.

4 H2O → 4 H+ + 4OH
4OH → 2H2O + O2 ↑ + 4e
      Mn, Cl, Z complex

➢ Assimilatory powers [ATP and NADPH] are formed in non-cyclic photophosphorylation. These assimilatory powers are used in dark reaction. The assimilatory powers are for fixation of CO2 into glucose.
➢ Photophosphorylation is the process in which ADP ( Adenosine Diphosphate ) is converted into ATP ( Adenosine Triphosphate ) by the addition of one phosphate ( Pi ) group, i.e inorganic phosphate, utilizing the energy from photons.
                                                                        ADP + Pi ATP
➢ Dark reaction (Biosynthetic phase) or Blackman reaction involves the fixation and reduction of CO2 resulting in the formation of carbohydrates.
➢ The dark phase occurs in the stroma of the chloroplast where all the enzyme necessary for CO2 fixation and synthesis of sugar and starch are located.
➢ RuBP (Ribulose bisphosphate) acts as a primary acceptor of atmospheric Co2 in the beginning of this phase and by the utilization of ATP and NADPH (products of light reaction) glucose is synthesized and RuBP is regenerated. The overall reaction of this phase is

Notes Photosynthesis ICSE Class 10 Biology

6 RuBP + 6 CO2 + 18 ATP + 12e → 6 RuBP + C6 H12O6 + 18 ADP + 18 Pi + 12 NADP + 6H2O
The hydrogen ions combine with CO2 to form glucose.
➢ Glucose produced during photosynthesis is soluble in water & consists of small molecules. As soon as glucose is produced. It is converted into starch by the process of polymerisation. The starch is insoluble in water. It is used for the storage of food in the plant cells.
➢ Adaptation of a leaf for photosynthesis – Large surface area to maximize light harvesting.

– Thinness of leaves to reduce distance for CO2 to diffuse through the leaf and to ensure light penetrates into the middle of the leaf.
– Arrangement of chloroplast on the upper surface of leaves so as to receive maximum amount of light.
– Presence of more stomata to allow rapid exchange of gases ( CO2 and O2 ).

Different Experiments and Factors Affecting Photosynthesis

Quick Review
Test for photosynthesis : Leaf is killed in boiling water ( 5-10 minutes ), dried, decolourised in warm spirit, moistened and dipped in iodine solution, Blue colour indicates starch.
CO2 is necessary for photosynthesis/Moll’s half experiment : One half of destarched leaf is inserted in air tight wide mouthed bottle having small quantity of KOH ( for absorption of CO2) and illuminated. Starch test after one hour indicates absence in inserted half and presence in outer half ( where CO2 is available ).
Light is necessary for photosynthesis : Intact leaf of a destarched plant is fitted in Ganong’s light screen with a designed cut in its lid. The same is exposed to light for some time and then tested for starch. Only the design through which light falls on the leaf becomes blue coloured.
Chlorophyll is essential for photosynthesis : Illuminated variegated leaf of Coleus/Photos is tested for starch. Only those areas turn blue which had chlorophyll.

Factors affecting Photosynthesis

External Factors
Light : Photosynthesis is successfully accomplished in the visible light (380 – 760 nm wave-length) of the spectrum. Rate of photosynthesis is maximum in red light, average in blue light and minimum in green light. A moderate light intensity is favourable for high rate of photosynthesis.
Carbon dioxide : 0.03 % CO2 is present in atmosphere. Increase in CO2 concentration upto 0.9% increases the rate of photosynthesis but concentration above 0.9% is harmful and decreases the rate of photosynthesis.
Temperature : Generally the photosynthesis increases with an increases in temperature in the range of 10-35°C. Beyond the 35°C the rate of photosynthesis decreases.
Water : About 1% total water absorbed is used in photosynthesis. Water deficiency reduces the rate of photosynthesis.

Internal Factors
Chlorophyll : It is essential for photosynthesis to occur. The rate of photosynthesis per unit of chlorophyll decreases with the age of leaf.
Accumulation of end product of photosynthesis – The rate of photosynthesis falls with the accumulation of food synthesized by photosynthesis.

Know the Terms

Photo-oxidation : The reaction of a substance with oxygen under the influence of light.
Accessory pigments : These are the pigments other than chlorophyll present in photosystems which help in capture light and pass it to the photocentres.
➢ CAM plants : These refers to the succulents and some other plants which show crassulacean acid metabolism.
Chemosynthesis : The process of synthesis of food in which the organisms use chemical reactions to obtain energy from inorganic compounds.
Photocentre : It is a primary pigment molecule in the photosystem where energy is used to derive of chemical reactions.
Photorespiration : It is highly wasteful, light dependent, utilization of oxygen and release of carbon-dioxide without release of energy.
Photosystem : It is light harvesting system present in the thylakoids of chloroplast.
➢ Photosynthetically Active Radiation ( PAR ) – It is visible spectrum of light between 400 nm to 700 nm.
Photolysis : It is splitting of water molecules in the chloroplast in presence of light.
Translocation : It is the process of movement of food from leaves to roots via phloem.
Autotrophs : Organisms capable of synthesizing their required nutrients from simple inorganic compounds in the presence of solar energy and chlorophyll.
Heterotrophs : An organism that cannot synthesize its own food and must feed on nutrients manufactured by autotrophs.
Carotenoids : A group of yellow, orange and red pigments found mostly in plastids.
Fluorescence : Emission of light, usually visible, of wave-length different from that absorbed from irradiated materials or from impact of electrons.
Phosphorescence : It is delayed emission of long wave radiations by irradiate substances which continues for sometime after removal of irradiation source.
Reaction Centres : It is chlorophyll molecule which converts light energy into chemical energy by bringing about electrical charge separation. Thus chlorophyll molecules act as reaction centres.
Phytol : It is a carbon chain which is attached to porphyrin ring of chlorophyll like a tail. Chemically phytol is C20H39OH.
Solarisation : Destruction of chlorophyll due to high light intensity is called solarisation.
Compensation point : The light intensity at which rate of photosynthesis is equal to the rate of respiration ( in morning and evening ).

Autotrophic Nutrition in Plants

We know that all living organisms consume some form of nutrients to sustain life. Animals consume plants or other animals. Plants consume carbon dioxide and water from the environment to produce food.

Therefore, the process of taking in a source of energy (food) from outside the body of an organism to inside is known as nutrition.

Do you know what mode of nutrition is carried out in plants? Plants have an autotrophic mode of nutrition. The term ‘autotrophic’ is derived from the Greek word ‘Auto’ meaning self and ‘troph’ meaning nutrition.

In this mode of nutrition, plants prepare or synthesize their own food with the help of inorganic raw materials. Thus, they are known as autotrophs.

Let us explore how plants prepare their own food.


Energy is essential for all life processes. All living organisms require nutrition. What is the ultimate source of nutrition on Earth?

The sun is the ultimate source of energy on Earth. Energy from the sun is captured by plants and converted into usable form. Thus, the origin of all foods is the food prepared by plants. This food is also consumed by animals.

Autotrophs such as green plants and some bacteria prepare or synthesize their own food. They are capable of trapping solar energy with the help of a green pigment called chlorophyll. This trapped solar energy is then converted into chemical energy of food using CO2 and H2O.

Photosynthesis is the process by which chlorophyll-containing cells present in leaves synthesize food in the form of carbohydrates by using carbon dioxide, water and sunlight.

Therefore, the raw materials required for photosynthesis are CO2 and H2O and the products formed are carbohydrates and O2. Hence, the process can be represented as:

Let us discuss the raw materials required for photosynthesis.

How are raw materials consumed by plants?

The raw materials required for photosynthesis are CO2 and H2O and the products formed are carbohydrates and O2.

Entry of raw materials
Plants obtain water through their roots. Water is then transported to all plant parts with the help of the xylem.
Exchange of gases (entry of CO2 and release of O2) occurs through the stomata.

Stomata are tiny pores present mainly on the surface of leaves. They are also present on the surface of young stems and roots.

Notes Photosynthesis ICSE Class 10 Biology

Stomata consist of a stomatal opening or stoma, which is surrounded by two distinct epidermal cells known as guard cells. The opposing inner walls of the guard cells are thick and inelastic. The remaining walls are thin and elastic.

Theories behind Stomatal Opening and Closing

Notes Photosynthesis ICSE Class 10 Biology

How do plants control the opening and closing of the stomata?

Two phases of photosynthesis

The process of photosynthesis occurs in two phases – Photochemical Phase and Biosynthetic Phase.

Photochemical Phase – A series of chemical reactions take place in the presence of light, as light behaves as catalyst is called as photochemical phase. The light reactions take place in thylakoids of the chloroplasts.

Light reactions – As the name suggests, this reaction takes place in the presence of light. Light energy is absorbed by chlorophyll molecules and is utilized for splitting water molecules into hydrogen and oxygen. Additionally in this phase, assimilatory power in the form of ATP and NADPH2 are produced. Light reactions occur in the membranes of thylakoids.

Events occurring during light reactions:
Absorption of light energy by chlorophyll molecules
Splitting of water molecules into hydrogen and oxygen atoms
Formation of ATP and NADPH2

Reactions involved in Photolysis:-

Notes Photosynthesis ICSE Class 10 Biology

Biosynthetic Phase – It includes the reactions that are not dependent on light (but may happen during day time as well). It results in synthesis of carbohydrates, or ‘food’, using the energy produced through light reactions.

Dark reactions – This reaction does not require direct light and occurs in the stroma of chloroplasts. During this phase, ATP and NADPH2 (formed during light reactions) are utilized for the reduction of CO2 to carbohydrates (food).

Event occurring during dark reaction: Reduction of CO2 to form carbohydrates Transformation of glucose molecules to 1 mole of Starch is called polymerisation.

Notes Photosynthesis ICSE Class 10 Biology

Some interesting facts:
Do you know that the total amount of O2 produced by an acre of trees per year is equal to the amount consumed by around 18 people annually!
One tree produces nearly 260 pounds of O2 annually.
Hydrogen is a clean fuel. Some green algae such as Chlamydomonas reinhardtii are being cultured to convert water into O2 and H2. This mass production of hydrogen could prove to be beneficial, but is still under research.

End Results of Products Of Photosynthesis
• Glucose: Simple glucose is utilised by plants in the following ways:
• For consumption by plant cells
• For storage as insoluble starch
• For conversion into sucrose
• For synthesis of fats, proteins, etc
• Water: It can be re-utilised in the continuance of photosynthesis.
• Oxygen: Some of it is used in respiration of leaves and rest diffuses out.

Global warming

Do you know that global warming can be reduced by growing more plants?

Green plants, as we know, utilize CO2 and water to produce food and in the process, release O2 gas. Thus, green plants help in reducing the amount of CO2 in the atmosphere. CO2 is a green house gas, which is one of the reasons for global warming.

Chloroplast- The site of Photosynthesis

Site of Photosynthesis
• Mesophyll cells in green leaves have large number of chloroplasts, which are the site of photosynthesis.
• Chloroplast consists of the grana and the stroma lamellae (forming a membranous system) and the fluid stroma.
• Membrane system of chloroplast − traps light, and synthesises ATP and NADPH (site of light-dependent reaction of photosynthesis)
• Stroma − CO2 is incorporated into the plant by enzymatic reactions, leading to the synthesis of sugar (site of light-independent reaction of photosynthesis)
• Chloroplasts are aligned along the walls of mesophyll cells so as to get optimum light.

Notes Photosynthesis ICSE Class 10 Biology

Pigments Involved in Photosynthesis
• An absorption spectrum is the graph plotted against the fraction of light absorbed by the pigment.
• An action spectrum is the rate of a physiological activity plotted against the wavelength of light.
• The similarity of the action spectrum of photosynthesis and the absorption spectrum of chlorophyll tells us that chlorophylls are the most important pigments in the process.

Notes Photosynthesis ICSE Class 10 Biology

(A) Absorption spectrum of chlorophylls a, b, and carotenoids
(B) Action spectrum of photosynthesis
(C) Action spectrum superimposed on absorption spectrum of chlorophyll a
4 types of pigments may be present in leaves: • Chlorophyll a (blue-green)
Chlorophyll b (yellow-green)
• Xanthophylls (yellow)
Carotenoids (yellow to yellow-orange)
Chlorophyll a is the main pigment in photosynthesis.
In VIBGYOR spectra, chlorophyll a shows maximum absorption, and hence, the rate of photosynthesis is the highest at the blue and red regions.
Accessory pigments: Chlorophyll b, xanthophylls and carotenoids
Absorb a wider range of light, and transfer the energy to chlorophyll a
Protect chlorophyll a from photo-oxidation

Factors Affecting Rate of Photosynthesis

Photosynthesis is influenced by internal (plant) factors and external factors.
• Internal factors: Number, size and orientation of leaves, mesophyll cells and chloroplasts, internal carbon dioxide concentration and the amount of chlorophyll.
• External factors: Availability of sunlight, temperature, carbon dioxide concentration and water.
• Law of Limiting Factors − If a chemical process is affected by more than one factor, then its rate will be determined by factor which is nearest to its minimal value (factor which directly affects the process if its quantity is changed).
This law of limiting factor was given by Blackman (1905).
• Light
• Incident light ∝ CO2 fixation rate; but at higher light intensities, the rate does not increase further as other factors become limiting
Light is rarely a limiting factor (with exception of the shade plants or plants of dense forest) because light saturation occurs at 10% of the full sunlight.
Beyond a point, if incident light is increased, then it leads to decrease in photosynthesis due to breakdown of chlorophyll.

• CO2 Concentration
• Major limiting factor
• Usually low in atmosphere (0.03 − 0.04%)
• Up to 0.05% − increases rate of CO2 fixation
• > 0.05% − damaging effect
• Though both C3 and C4 show increase in rate of photosynthesis at high light intensities accompanied by high CO2 concentration. The saturation point for C3 is obtained at higher concentrations as compared to C4. Therefore, CO2 concentration is more of a limiting factor for C3 plants.
• Increased CO2 concentration is beneficial for greenhouse crops such as tomatoes and bell pepper.

• Temperature
• Dark reactions are more sensitive to an increase in temperature.
• C4 plants respond more to an increase in temperature and show higher rate of photosynthesis as compared to C3 plants.
• Adaptations according to habitat also affect temperature optimum for photosynthesis. Tropical plants have higher temperature optimum compared to plants growing in temperate climates.

• Water
• Water stress causes stomata to close and hence, less CO2 is available.
• Water stress causes the leaves to wilt, thereby reducing their surface area and metabolic activity as well.

Experiments Related to Photosynthesis

We know that raw materials are utilized by plants to prepare food. Do plants prepare food at all times? Are there any essential conditions required for photosynthesis?

1. Sunlight is essential for photosynthesis

Notes Photosynthesis ICSE Class 10 Biology

Place a healthy green potted plant in a dark room for 1-2 days. This is done to ensure that the plant consumes all its reserve food and the leaves do not contain any starch. Then, cover a portion of a leaf of this plant on both sides with two uniform pieces of black paper, fixed in position with two paper clips.

Now, expose this plant to bright light. After a few hours, remove the leaf, decolourize it with alcohol, and test the presence of food (starch) with iodine solution.

You will observe that the portion of the leaf covered with black paper does not show any presence of starch (food).

Explanation of the activity:

The food prepared by plants (carbohydrates) through the process of photosynthesis is stored as starch. This starch reacts with the iodine solution to change to blue-black colour. Only those portions of the leaf that were exposed to sunlight could photosynthesise and hence, change to blue-black colour when tested with iodine.

2. Chlorophyll is essential for photosynthesis

Place a variegated plant (i.e., a plant which has both green and non-green areas, for e.g., croton or money plant) in a dark room for 2 – 3 days. This is done to ensure that all the reserve food (starch) is utilized.

Notes Photosynthesis ICSE Class 10 Biology

Place this plant in sunlight for six hours to allow photosynthesis to take place. Then, pluck a leaf from this plant and trace the green areas on a sheet of paper.

Now, decolourize the leaf using alcohol and dip it in a dilute solution of iodine for a few minutes. Wash this leaf with water and compare it with the tracings of the leaf done earlier. It will be observed that only the green areas of the leaf could photosynthesize.

The leaf is treated with alcohol so that it loses its green colour (chlorophyll pigment) and blue-black colour (in presence of starch) obtained after treatment with iodine.

The green parts of a variegated leaf contain chlorophyll. Therefore, only these parts could photosynthesize and manufacture food. Thus, the change in colour was observed only in these parts.

3 CO2 is essential for photosynthesis

Notes Photosynthesis ICSE Class 10 Biology

Select two healthy potted plants of nearly the same size and label them as A and B. Place them in a dark room for 2-3 days. Then, place two glass plates under both the plants. Place a watch-glass containing potassium hydroxide besides pot A. Cover both the plants by inverting separate bell jars over them. Potassium hydroxide, as we know, is used to absorb CO2. Therefore, CO2 is not available for plant A.

Now, seal the bottom of the jars to the glass plates with the help of Vaseline. This prevents the entry of CO2 into the set up. Then, place the plants under sunlight for 2 – 3 hours. Test one leaf each from both plants for the presence of starch, using alcohol and iodine (as explained in the previous activity). It will be observed that plant B has a higher amount of starch as compared to plant A

Explanation of the activity: 

This happens because potassium hydroxide present besides plant A absorbs all the CO2. Therefore, plant A is not able to photosynthesize and manufacture food. Hence, the amount of starch present in plant B is higher than plant A.

Photosynthesis in a laboratory

Place an aquatic plant (hydrilla) in a beaker filled with water. Cover the plant with a transparent funnel. Then, invert a test tube over the open end of the funnel.

Notes Photosynthesis ICSE Class 10 Biology

While inverting the test tube, make sure it does not contain any air bubbles. Place this apparatus in sunlight and observe the changes.

It will be observed that after sometime, air bubbles (O2) emerge in the test tube.

Importance of Photosynthesis and Carbon Cycle

Adaptations in Leaves for Photosynthesis

Leaves often exhibit a number of adaptations to increase the rate of photosynthesis. Some of them are as follows:

 Large surface area for maximum sunlight absorption
 Leaf arrangement at correct angle for maximum light
 Transparent cuticle and upper epidermis to allow free entry of light
 Large number of stomata for maximum exchange of gases
 Thin leaves to reduce the distance between cells involved in rapid transportation
 Chloroplasts concentrated at the upper layer to absorb light quickly
 Extensive vein system for rapid transportation

Importance of Photosynthesis
• Provides food- Photosynthesis is the basis for production of food by the autotrophs, i.e. plants. All other organisms are directly or indirectly dependent on the food produced by plants for their survival.
• Provides oxygen- Oxygen is produced during photosynthesis which is the life supporting gas. All organisms are dependent on the oxygen to sustain their life.

Carbon Cycle
Carbon cycle is a series of chemical reactions in which carbon as a chemical element gets consumed by living organisms and again gets restored in the atmosphere by various means.

Notes Photosynthesis ICSE Class 10 Biology

The steps involved in carbon cycle are:
• Autotrophs use carbon dioxide to produce carbohydrates through photosynthesis.
• These carbohydrates keep travelling from one to another organism through food chain.
• Plants and animals respire by oxidising carbohydrates to produce energy.
• In the process of decomposition, the bacteria break down the inorganic matter to release carbon dioxide back into atmosphere.
• Combustion also releases the carbon dioxide stored in fuels back to the atmosphere.

Photosynthesis ICSE Class 10 Biology